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KMID : 0381920040340010031
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Korean Journal of Microscopy
2004 Volume.34 No. 1 p.31 ~ p.42
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Effect of PLA2 Inhibitor Rutin on Endotoxin-Induced Acute Lung Injury
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Kim Seong-Eun
Lee Young-Man
Park Won-Hark
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Abstract
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Acute respiratory distress syndrome (ARDS) is a kind of acute lung injury characterized by inflammatory disruption of alveolar-capillary barrier and notorious for its high mortality. Neutrophils cause cell damage through the production of free radicals, inflammatory mediators, and proteases in ARDS. PLA2 might serve a primary regulatory role in the activation of neutrophils. This present study was performed to elucidate the effect of rutin known as PLA2 inhibitor on ARDS induced by endotoxin. Endotoxin had increased lung myeloperoxidase (MPO) activity, BAL (bronchoalveolar lavage) protein content, numbers of neutrophils in BALF (bronchoalveolar lavage fluid) compared with those of control rat (p<0.001). In addition, histological evidence of lung injury was correlated with neutrophil influx into alveolar space and cerrous perhydroxide granules were found in lining of endothelial cell, alveolar type I, II cells. In contrast, pretreated group of rutin had significantly decreased all of the parameters (p<0.001). These data suggest that inhibition of PLA2 is one step approach that block the process of ARDS. Accordingly, we conclude that rutin can be used as the prophylactic agent for ARDS on the bases of these experimental results.
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KEYWORD
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Acute lung injury , ARDS , Endotoxin , Neutrophils , Rutin
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